kidney cancer renca  (Procell Inc)

Journal: Journal of Nanobiotechnology

Article Title: Finely tuned ionizable lipid nanoparticles for CRISPR/Cas9 ribonucleoprotein delivery and gene editing

doi: 10.1186/s12951-024-02427-2

Figure Lengend Snippet: Effect of pH on the uptake and gene editing efficiency of CrLNPs. Confocal microscopy of A , B HEK293T cells and C , D various cell lines (CT26, 4T1, DCON, RENCA, Raw264.7) treated with CrLNPs to examine cellular uptake (scale bar: 10 μm for A , 40 μm for C ). B , D Quantification of fluorescence by image analyses. Data are shown as mean ± SD, * P < 0.05, *** P < 0.001, **** P < 0.0001 by student’s t -test. E Schematic of CrLNPs treatment to HEK293T cells expressing surrogate reporter and analyses by flow cytometry. Gene editing efficiencies of HEK293T determined by F flow cytometry and G confocal microscopy. Scale bar, 40 μm

Article Snippet: CT26 murine colon cancer cells, 4T1 murine breast cancer cells, RENCA murine kidney cancer cells, Raw264.7 murine macrophage cells, and HEK293T human embryonic kidney cells were obtained from the American Type Culture Collection (ATCC).

Techniques: Confocal Microscopy, Fluorescence, Expressing, Flow Cytometry

Journal: Journal of medical virology

Article Title: Swine pseudorabies virus attenuated vaccine reprograms the kidney cancer tumor microenvironment and synergizes with PD-1 blockade.

doi: 10.1002/jmv.29568

Figure Lengend Snippet: FIGURE 1 The oncolytic activity of Pseudorabies virus live attenuated vaccine(PRV‐LAV) against kidney cancer in vitro. (A) Cell morphology of A498, Caki‐1, 786‐0, and Renca cells 48 h postinfection with PRV‐LAV. Phase‐contrast micrographs were captured using a Thermo EVOS M7000. Scale bars, 100 μm. (B) Cell viability assays were performed on A498, Caki‐1, 786‐0, and Renca cells 72 h after exposure to PRV‐LAV. Data are presented as the mean ± s.d. values (n = 5). A t‐test was used to determine the significance of differences between the PRV‐LAV infection group and the Mock group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (C) The expression of PRV gB in the infected cells was detected by immunofluorescence assay. Graphs were captured by Leica DMI8. Scale bars, 250 μm. (D) The expression of PRV gB was analyzed by western blot analysis after cancer cells were infected with PRV‐LAV (MOI = 1) and cultured for 0, 12, 24, 36, and 48 h. GAPDH was used as a loading control. Virus titer in the culture supernatant of A498 (E), Caki‐1 (F), 786‐0 (G), and Renca (H) cells post‐PRV‐LAV infection. Data are presented as the mean ± s.d. values (n = 4). PRV‐LAV, Pseudorabies virus live attenuated vaccine.

Article Snippet: Human kidney cancer cell line A498 (HTB‐44) and mouse kidney cancer cell line Renca (CRL‐2947) were purchased from the American Type Culture Collection (ATCC).

Techniques: Activity Assay, Virus, In Vitro, Infection, Expressing, Immunofluorescence, Western Blot, Cell Culture, Control

Journal: Journal of medical virology

Article Title: Swine pseudorabies virus attenuated vaccine reprograms the kidney cancer tumor microenvironment and synergizes with PD-1 blockade.

doi: 10.1002/jmv.29568

Figure Lengend Snippet: FIGURE 2 The safety and therapeutic activity of Pseudorabies virus live attenuated vaccine (PRV‐LAV) against kidney cancer in vivo. (A) Timeline of the experimental setup for the experiments in the BALB/c mouse model. Weight curve (B) and survival rate curve (C) of mice after intravenous injection of vehicle or PRV‐LAV (4 × 107 PFUs) into the tail vein. Data are presented as the mean ± s.d. values (n = 6). (D) Timeline of the experimental setup for the experiments in the BALB/c nude mouse model. (E) Tumor volume curves of mice bearing A498 tumors treated with vehicle or PRV‐LAV (1 × 107 PFUs, intratumorally). Data are presented as the mean ± s.d. values (n = 6). (F) Timeline of the experimental setup for the experiments in the Renca syngeneic models. (G) Tumor volume curves of mice bearing Renca tumors treated with vehicle or PRV‐LAV (1 × 107 PFUs, intratumorally) with an initial tumor volume of approximately 100mm3. Data are presented as the mean ± s.d. values (n = 5). (H) Timeline of the experimental setup for the experiments in the Renca syngeneic models. (I) Tumor volume curves of mice bearing Renca tumors treated with vehicle or PRV‐LAV (1 × 107 PFUs, intratumorally) with an initial tumor volume of approximately 200 mm3. Data are presented as the mean ± s.d. values (n = 5). In (E, G, I), comparisons were performed by AUC analysis. Statistical analysis was performed by t‐test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. PRV‐LAV, Pseudorabies virus live attenuated vaccine.

Article Snippet: Human kidney cancer cell line A498 (HTB‐44) and mouse kidney cancer cell line Renca (CRL‐2947) were purchased from the American Type Culture Collection (ATCC).

Techniques: Activity Assay, Virus, In Vivo, Injection